Fig. 5. Type I collagen prevented ·OH induced-apoptosis in HeLa cells. Apoptosis was detected by DAPI staining. (a) Normal control without ·OH treatment (b) Apoptosis-positive control; HeLa cells were treated with 0.1 mM FeSO4/0.6 mM H2O2 and incubated for 24 h. (c) Collagen-pretreatment group; HeLa cells were pretreated with type I collagen for 2 h, then treated with 0.1 mM FeSO4/0.6 mM H2O2 an..